The content of the total protein, protein fractions and blood serum proteins in patients with different forms of lichen ruber planus

  • N. G. Barannik State institution "Zaporizhia Medical Academy of Post-Graduate Education Ministry of Health of Ukraine"
  • R. Y. Tsymbaliuk State institution "Zaporizhia Medical Academy of Post-Graduate Education Ministry of Health of Ukraine"
  • O. M. Manukhina State institution "Zaporizhia Medical Academy of Post-Graduate Education Ministry of Health of Ukraine"
  • A.V. Sydoryako State institution "Zaporizhia Medical Academy of Post-Graduate Education Ministry of Health of Ukraine"
  • K. P. Volchykhina State institution "Zaporizhia Medical Academy of Post-Graduate Education Ministry of Health of Ukraine"
Keywords: Lichen Ruber Planus, mixed saliva, total protein content, inhibitor proteinase

Abstract

Purpose of the study. Establishing the role of processes of proteolysis of mixed saliva in the development and course of lichen planus of the oral mucosa.

Materials and methods. A comprehensive examination of 102 patients with lichen planus aged 21 to 70 years and 20 people in the control group, whose age and sex composition corresponded to that in the study group. BioRad (USA) reagent kits were used to determine the total protein content of mixed saliva. The content of protein fractions of mixed saliva was determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Determination of serum proteins in mixed saliva was performed by quantitative (cross) immunoelectrophoresis.

Results. In patients with lichen planus, the overall proteolytic activity of mixed saliva increases with a significant increase in the concentration of α1proteinase inhibitor, especially in exudative hyperemic and erosiveulcerative forms of the disease. Diffusion of α1proteinase inhibitor into mixed saliva increases its antiproteolytic potential and has a protective character. The content of albumin and ceruloplasmin in the mixed saliva of patients with lichen planus increases depending on the severity of the disease: typical, hyperkeratotic, exudativehyperemic, erosiveulcerative.

Conclusions. Mixed saliva of patients with lichen planus in contrast to patients in the control group is characterized by the predominance of low molecular weight proteins (20–79 kDa) over high molecular weight. The level of albumin, α1proteinase inhibitor and ceruloplasmin in the mixed saliva of patients with lichen planus increases and correlates with the severity of the disease. The content of IgA in the mixed saliva of patients with lichen planus increases, depending on the form of the disease.

References

Oberti L, Gabrione F, Lucchese A, Di Stasio D, Carinci F and Lauritano D (2019). Treatment of orallichen planus: a narrative review. Front. Physiol. Conference Abstract:5th National and1st International Symposium of Italian Society of Oral Pathology and Medicine. doi: 10.3389/conf.fphys.2019.27.00004.

Kalaskar AR, Bhowate RR, Kalaskar RR, Walde SR, Ramteke RD, Banode PP. Efficacy of herbal interventions in oral lichen planus: A systematic review. Contemp Clin Dent 2020; 11: 311–9.

Gupta J, Aggarwal A, Asadullah MD, Khan MH, Agrawal N, Khwaja KJ. Vitamin D in the treatment of oral lichen planus: A pilot clinical study. J Indian Acad Oral Med Radiol 2019; 31: 222–7.

Hamour AF, Klieb H, Eskander A. Oral lichen planus. Canadian Medical Association Journal [Internet]. 2020;192(31):E892-E892. Available from: doi:10.1503/cmaj.200309.

Cosgarea R, Pollmann R, Sharif J, Schmidt T, Stein R, Bodea A, et al. Photodynamic therapy in oral lichen planus: A prospective case-controlled pilot study. Scientific Reports [Internet]. 2020;10(1): Available from: doi:10.1038/s41598-020-58548-9.

Ertugrul AS, Arslan U, Dursun R, Hakki SS (2013) Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis. Int J Oral Sci 5: 92–97.

Wang L, Yang Y, Xiong X, YuT, Wang X, Meng W, Wang H, Luo G, Ge L (2018) Oral lichen-planus-associated fibroblasts acquire myofibroblast characteristics and secrete pro-inflammatory cytokines in response to Porphyromonas gingivalis lipopolysaccharide stimulation. BMC Oral Health 18: 197.

Eliseeva OV, Sokolova II. Lechenii bolnyih generalizovannyim parodontitom na fone krasnogo ploskogo lishaya lizotsim soderzhaschimi lekarstvennyimi preparatami.VIsnik problem bIologIYi ta meditsini.2015; 2:2 (119):83–89.

Bazhina II, Koshkin SV, Zaytseva GA. Harakter izmeneniy immuno- logicheskih pokazateley u patsientov s krasnyim ploskim lishaem. Eksperimentalnaya meditsina i klinicheskaya diagnostika. Vyatskiy meditsinskiy vestnik.2017: 3(55).

Brodovska NB. Stan prooksidantnoYi sistemi krovI ta endogennoYi IntoksikatsIYi u hvorih na chervoniy ploskiy lishay. Bukovinskiy medichniy vIsnik. 2017; 21: 4 (84).

Talungchit S, Buajeeb W, Lerdtripop C, Surarit R, Chairatvit K, Roytrakul S, Kobayashi H, Izumi Y, Khovidhunkit SP (2018) Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study. BMC Oral Health 18: 42.

VillaTG, Sánchez-Pére Á, Sieiro C.Oral lichen planus: a microbiologist point of view. Int Microbiol (2021). https://doi.org/10.1007/ s10123-021-00168-y.

Romanenko IG, Gorobets SM, Salischeva VO. Sovremennyiy vzglyad na problemu lecheniya krasnogo ploskogo lishaya (obzor literaturyi).Vestnik meditsinskogo instituta «REAVIZ».2019;6.

Aleksandrova KV, KrIsanova NV, Rudko N P. ObmIn prostih bIlkIv v normI ta pri patologIYi: navchalniy posIbnik dlya studentIv 2 kursu medichnih fakultetIv. ZaporIzhzhya[ZDMU]; 2020.140p.

Tsimbalyuk RYu. KlInIka, dIagnostika ta lIkuvannya chervonogo pleskato- go lishayu slizovoYi obolonki porozhnini rota. [Avtoreferat disertatsIYi na zdobuttya naukovogo stupenya kandidata medichnih nauk]. KiYiv ;2006.

Zhukov VI, Gorbach TV, Denisenko SA. Ukl. BIohImIya zuba I slini: Metod. ukaz. dlya studentIv stomatologIchnogo fakultetu 2 kursi.HarkIv: HNMU;2012. 40 p

Published
2021-07-20
How to Cite
Barannik, N. G., Tsymbaliuk, R. Y., Manukhina, O. M., Sydoryako, A., & Volchykhina, K. P. (2021). The content of the total protein, protein fractions and blood serum proteins in patients with different forms of lichen ruber planus. Modern Medical Technology, (2(49), 31-36. https://doi.org/10.34287/MMT.2(49).2021.5
Section
Original research