Characteristics of the mechanisms of anti-inflammatory action of cryopreserved placenta extract and diclofenac sodium by their threaded administration
Introduction. Inflammation is a complex multicomponent adaptive pathological process based on three enzymatic pathways of arachidonic acid metabolism: cyclooxygenase, lipoxygenase and epoxygenase. Nonsteroidal anti-inflammatory drugs are the most numerous and most widely used group of drugs used in the pharmacocorrection of inflammatory processes of various etiologies.
Purpose of the study. The aim is to characterize the effect of cryopreserved placenta extract on the anti-inflammatory activity of diclofenac sodium with their threaded administration in a model of zymosan-induced inflammation.
Materials and methods. In vivo experimental studies were performed on 28 nonlinear laboratory male rats weighing 200–220 g. Acute exudative inflammation was reproduced by subplantar administration of rat limb 0,1 ml of 2,0% zymosan suspension. The anti-exudative effect was assessed by the magnitude of limb edema, which was assessed using an aqueous plethysmometer.
Results and discussion. The study showed that subplantar administration of 2,0% suspension of zymosan led to a statistically significant (p < 0,05) increase in the volume of the damaged limb after 30 minutes. by 28,1 ± 5,4% relative to baseline and was 2,01 ± 0,06 ml. The most pronounced and almost comparable anti-inflammatory activity was observed against the background of the use of cryopreserved placenta extract and the combined use of diclofenac sodium and cryopreserved placenta extract. Thus, for 60 min the antiinflammatory activity was (p < 0,05) 46,5% and 53,2%, respectively.
Conclusions. It is established that one of the leading mechanisms of anti-inflammatory activity of cryopreserved placenta extract is the inhibition of the lipoxygenase pathway of arachidonic acid metabolism. This was indicated by statistically significant (p < 0,05) suppression of zymosaninduced inflammation in rats by 78,8% and 74,8% by 120 and 180 min, respectively.
Karateev AE, Aleinikova TL. Eicosanoids and inflammation. Modern Rheumatology Journal. 2016; 10 (4): 73–86. DOI: http:// dx.doi.org/10.14412/1996-7012-2016-4-73-86.
Karateev AE, Nasonov EL, Ivashkin VT and others; Association of Rheumatologists of Russia, Russian Society for the Study of Pain, Russian Gastroenterological Association, Russian Scientific Medical Society of Therapists, Association of Traumatologists and Orthopedists of Russia, Russian Association of Palliative Medicine. Rational use of nonsteroidal anti-inflammatory drugs. Clinical recommendations. Scientific and practical rheumatology. 2018; 56: 1–29. DOI: http:// doi.org/10.14412/1995-4484-2018-1-29.
Hladkykh FV. Preventive and treatment strategies for pharmacocorrection of gastropathy induced by nonsteroidal anti-inflammatory drugs. Reviews of clinical pharmacology and drug therapy. 2017; 4: 14–23. DOI: http://dx.doi.org/10.17816/ RCF15414-23.
Hladkykh FV, Chyzh MO. Nonsteroidal antiinflammatory drugs: a modern understanding of the mechanisms of damage to the digestive tract, the shortcomings of pathogenetic drugs and prospects for biological therapy of NSAIDinduced esophagogastroenterocolonopathy. Gastroenterology. 2020; 4: 253–66. DOI: https:// doi.org/10.22141/2308-2097.54.4.2020.216714.
Goltsev AN, ed. Placenta: cryopreservation, clinical use. Kharkiv; 2013. 268 p.
Pogozhykh O, Prokopyuk V, Figueiredo C, Pogozhykh D. Placenta and placental derivatives in regenerative therapies: experimental studies, history, and prospects. Stem Cells International. 2018; 2018: 1–14. DOI: https://doi. org/10.1155/2018/4837930.
Hladkykh FV, Chyzh MO. Modulation of meloxicam-induced changes in secretory and motor activity of the stomach by using placental cryoextract. Proceeding of the Shevchenko Scientific Society. Medical sciences. 2021; 64 (1): 84–94. DOI: https://doi.org/10.25040/10.25040/ ntsh2021.01.08 Available from: https://mspsss. org.ua/index.php/journal/article/view/400.
Hladkykh FV, Chyzh MO. The effect of cryoirrigation and cryopreserved placenta extract on the content of nitrogen monoxide in the gastric mucosa in rats with diclofenac sodium-induced gastropathy. The 58-th Annual Meeting of the Society for Cryobiology «CRYO 2021», 21–23 July, Chicago. 2021; Chicago. USA, 2021. p. 97–8.
Gryshchenko NG, Klimenko NA, Gorgol NI, Tatarko SV. Effect of placental cryoextract on chronic inflammation of the ovaries in mice. Medicine today and tomorrow. 2010; 2–3 (47–8): 7–17. Available from: http://dspace.nbuv.gov.ua/ handle/123456789/44519.
Stefanov OJ, ed. Preclinical studies of drugs: guidelines. Kyiv: Avicenna; 2001. 527 p.
Drogovoz SM, Belyk GV, Kudina OV, Demyanenko DV, Mohammad RD. Screening pharmacological studies of liquefied gas extracts in linden blossoms. Pharmaceutical Journal. 2012; 5: 94–100.
Sigidin YA, Arzamastsev AP, Lieberman SS, Schwartz GYa, ed. Drug therapy of the inflammatory process: experimental and clinical pharmacology of anti-inflammatory drugs: a monograph. Moscow: Medicine; 1988. 240 p.
Rybolovlev YuR, Rybolovlev RS. Dosage of substances for mammals by constants of biological activity. Reports of the USSR Academy of Sciences. 1979; 247 (6): 1513–6.
Fereidoni M, Ahmadiani A, Javan M. Semnanian S, An accurate and simple method for measurement of paw edema. Journal of Pharmacological and Toxicological Methods. 2000; 43 (1): 11–4. DOI: http://doi.org/10.1016/s1056- 8719(00)00089-7.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.